Kinsey J A, Fincham J R, Siddig M A, Keighren M
Genetics. 1980 Jun;95(2):305-16. doi: 10.1093/genetics/95.2.305.
The am locus of Neurospora codes for NADP-dependent glutamate dehydrogenase (GDH). Four new am mutants that produced mutationally altered GDH have been characterized. Mutant am119 is a CRM-negative, complementing mutant that maps between am2 and am1. The other three mutants are CRM formers that produce varieties of GDH that can be activated by glutamate or succinate. The GDH of am130 and am131 is similar in terms of activation properties to that of am3. The GDH of am122 requires very high concentrations of dicarboxylate for activity. The mutation in am130 maps between am14 and am2 and resulted in a replacement at residue 75 of the GDH (pro leads to ser). The mutation in am122 maps near am11 and apparently resulted in the replacement of the tryptophan residue at position 389 with an unknown amino acid. The mutation in am131 maps between am2 and am1.
粗糙脉孢菌的am基因座编码依赖NADP的谷氨酸脱氢酶（GDH）。已对四个产生突变型GDH的新am突变体进行了表征。突变体am119是一个CRM阴性的互补突变体，其定位在am2和am1之间。其他三个突变体是CRM形成者，它们产生的多种GDH可被谷氨酸或琥珀酸激活。am130和am131的GDH在激活特性方面与am3的相似。am122的GDH需要非常高浓度的二羧酸才能发挥活性。am130中的突变定位在am14和am2之间，导致GDH第75位残基发生替换（脯氨酸变为丝氨酸）。am122中的突变定位在am11附近，显然导致第389位的色氨酸残基被一个未知氨基酸取代。am131中的突变定位在am2和am1之间。
