Ueba O
Acta Med Okayama. 1980 Sep;34(4):245-54. doi: 10.18926/AMO/30521.
Treatment of respiratory syncytial (RS) virus with Triton X-100 in solutions of low ionic strength solubilized not only glycoproteins but also some non-glycosylated proteins. Rate zonal sedimentation of the solubilized materials resulted in separation of the glycoproteins from the other components, i.e. one fraction predominantly composed of two glycoproteins each with molecular weight (mol. wt.) of approximately 100,000 and 53,000 was obtained. Electron microscopic observation of this fraction revealed numerous club- or rod-shaped fine structures, suggesting that these were spikes of RS virus. After removal of Triton X-100 the structures aggregated and formed oligomers and polymers.
在低离子强度溶液中用曲拉通X-100处理呼吸道合胞（RS）病毒，不仅可溶解糖蛋白，还能溶解一些非糖基化蛋白。对溶解后的物质进行速率区带沉降，可将糖蛋白与其他成分分离，即得到一个主要由两种糖蛋白组成的组分，每种糖蛋白的分子量约为100,000和53,000。对该组分进行电子显微镜观察，发现有许多棒状或杆状的精细结构，表明这些是RS病毒的刺突。去除曲拉通X-100后，这些结构聚集形成寡聚体和聚合物。
