Schmitt H
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1980;215(2):79-86. doi: 10.1007/BF00414465.
In rabbit eyes there was made a central superficial defect into the cornea with a 7.2-mm Trepan/0.35 mm depth. To show alterations in the pattern of acid mucopolysaccharides during wound healing histochemical investigation was carried out in a series of toluidine blue preparations of pH 3,8-5,2 of unfixed frozen corneal sections. Table 1 shows the results of metachromasia after superficial keratectomy. On the first postoperative day metachromasia was extremely reduced in the area of keratectomia. Metachromasia in the surrounding cornea became normal after three days while in the area of corneal defect it lasted a long time, until the normal pattern of acid mucopolysaccharides is reconstructed. After three months the metachromasia was still reduced in the superficial parts of the corneal stroma (Fig. 3). After six months cornea was transparent in the area of ketatectomy with no alterations in the pattern of mucopolysaccharides. This showed the long alterations in the pattern of mucopolysaccharides. This showed the long healing procedure in bradytrophic corneal tissue.
在兔眼中，用7.2毫米环钻制作了一个深度为0.35毫米的角膜中央浅表缺损。为了显示伤口愈合过程中酸性黏多糖模式的变化，对一系列pH值为3.8 - 5.2的未固定冷冻角膜切片的甲苯胺蓝制剂进行了组织化学研究。表1显示了浅表角膜切除术后异染性的结果。术后第一天，角膜切除术区域的异染性极度降低。周围角膜的异染性在三天后恢复正常，而角膜缺损区域的异染性持续了很长时间，直到酸性黏多糖的正常模式重建。三个月后，角膜基质浅表部分的异染性仍然降低（图3）。六个月后，角膜切除术区域的角膜透明，黏多糖模式无变化。这表明黏多糖模式的长期变化。这表明营养不良性角膜组织的愈合过程漫长。
