Lydyard P M, Brostoff J, Pack S, Parry H F
Immunobiology. 1981;158(3):173-81. doi: 10.1016/S0171-2985(81)80067-8.
The T cell mitogen concanavalin A (Con A) was added to lymphocytes pre-cultured for 24 hours in vitro in tissue culture medium. Delayed addition of the mitogen resulted in an enhanced lymphocyte activation measured by incorporation of radioactive precursors of DNA. T cells isolated by rosetting with sheep erythrocytes also exhibited this enhancement. In a limited study, lymphocytes from both atopic patients and those with systemic lupus erythematosus (SLE) showed little or no enhanced responsiveness to Con A. The possibility that some patients with atopy and SLE possess defective suppressor T cells is discussed in the light of these data.
将T细胞促有丝分裂原刀豆球蛋白A（Con A）添加到在组织培养基中体外预培养24小时的淋巴细胞中。促有丝分裂原的延迟添加导致通过DNA放射性前体掺入测量的淋巴细胞活化增强。通过与绵羊红细胞进行玫瑰花结试验分离的T细胞也表现出这种增强。在一项有限的研究中，来自特应性患者和系统性红斑狼疮（SLE）患者的淋巴细胞对Con A的反应性几乎没有增强或没有增强。根据这些数据讨论了一些特应性和SLE患者存在抑制性T细胞缺陷的可能性。
