Sakane T, Takada S, Suzuki N, Tsuchida T, Murakawa Y, Ueda Y
J Immunol. 1986 Dec 15;137(12):3809-13.
Concanavalin A (Con A)-activated T lymphocytes from patients with active, but not inactive, systemic lupus erythematosus (SLE) failed to express normal suppressor activity, regardless of the phenotype of CD4+ or CD8+. Con A-activated CD4+ or CD8+ T lymphocytes from the SLE patients and from normal controls were further separated into two populations, using the autologous erythrocyte rosette technique. One population very rich in cells capable of forming rosettes with autologous erythrocytes from the active patients showed the same degree of suppressor activity, as did that from normal controls; the CD4+ or CD8+ population poor in autorosetting cells derived from Con A-activated T lymphocytes from both the controls and patients did not express suppressor activity. Moreover, when autorosetting T cells from the active patients and nonrosetting cells from the same patients were mixed at a normal ratio (4:6), normal suppressor activity could be restored. It was notable that the frequency of autorosette-forming cells was markedly reduced in the Con A-activated T lymphocytes from the active, but not inactive, SLE patients, regardless of the phenotype of CD4+ or CD8+. These findings indicate the presence of a normally functioning suppressor T cell population in patients with active SLE. It seems that the lack of suppressor T cell function in patients with active SLE is due to the dilution of a few normal suppressor T cells by large numbers of nonsuppressor T lymphocytes.
伴刀豆球蛋白A（Con A）激活的、来自活动期而非非活动期系统性红斑狼疮（SLE）患者的T淋巴细胞，无论其CD4⁺或CD8⁺表型如何，均不能表达正常的抑制活性。利用自体红细胞花环技术，将来自SLE患者和正常对照的Con A激活的CD4⁺或CD8⁺T淋巴细胞进一步分为两个群体。一个群体富含能够与活动期患者的自体红细胞形成花环的细胞，其显示出与正常对照相同程度的抑制活性；来自对照和患者的Con A激活的T淋巴细胞中，自体花环形成细胞较少的CD4⁺或CD8⁺群体不表达抑制活性。此外，当将活动期患者的自体花环形成T细胞与同一患者的非花环形成细胞以正常比例（4:6）混合时，可恢复正常的抑制活性。值得注意的是，无论CD4⁺或CD8⁺表型如何，来自活动期而非非活动期SLE患者的Con A激活的T淋巴细胞中，自体花环形成细胞的频率均显著降低。这些发现表明活动期SLE患者存在功能正常的抑制性T细胞群体。似乎活动期SLE患者抑制性T细胞功能的缺乏是由于少数正常抑制性T细胞被大量非抑制性T淋巴细胞稀释所致。
