MacDonald H R, Thiernesse N, Cerottini J C
J Immunol. 1981 May;126(5):1671-5.
The inhibitory effect of monoclonal anti-Lyt-2 antibodies on T cell-mediated cytolysis has been investigated at the clonal level. In agreement with previous reports from several laboratories, populations of cytolytic T lymphocytes (CTL) generated in vitro in mixed leukocyte cultures (MLC) were reversibly inhibited by monoclonal anti-Lyt-2 antibodies in a dose-dependent fashion. However, when alloimmune peritoneal exudate lymphocytes (PEL) were used as a source of CTL, little or no inhibitory effect of anti-Lyt-2 antibodies on cytolysis was observed. A series of CTL clones derived from MLC or PEL populations was also tested for inhibition of cytolysis by anti-Lyt-2 antibodies. In agreement with results obtained at the population level, most MLC-derived clones (81%) were strongly inhibited by the reagent, whereas few PEL clones (15%) were inhibited. Several of these clones were expanded and maintained in culture without loss of their "inhibition phenotype." Flow cytofluorometric analysis using the same monoclonal anti-Lyt-2 antibodies further revealed that both inhibited and uninhibited clones expressed comparable amounts of Lyt-2 antigen. These results provide direct evidence that inhibition of CTL by anti-Lyt-2 antibodies is heterogeneous at the clonal level. The possibility that this heterogeneity may be related to avidity of antigen receptors is discussed.
已在克隆水平上研究了单克隆抗Lyt-2抗体对T细胞介导的细胞溶解作用的抑制效果。与几个实验室先前的报道一致，在混合淋巴细胞培养（MLC）中体外产生的细胞毒性T淋巴细胞（CTL）群体，被单克隆抗Lyt-2抗体以剂量依赖方式可逆性抑制。然而，当同种免疫腹膜渗出淋巴细胞（PEL）用作CTL来源时，未观察到抗Lyt-2抗体对细胞溶解有明显抑制作用。还测试了一系列源自MLC或PEL群体的CTL克隆对细胞溶解的抑制情况。与群体水平获得的结果一致，大多数源自MLC的克隆（81%）被该试剂强烈抑制，而很少有PEL克隆（15%）被抑制。其中几个克隆在培养中得以扩增并维持，且未丧失其“抑制表型”。使用相同的单克隆抗Lyt-2抗体进行的流式细胞荧光分析进一步显示，被抑制和未被抑制的克隆表达的Lyt-2抗原量相当。这些结果提供了直接证据，表明抗Lyt-2抗体对CTL的抑制在克隆水平上是异质性的。本文讨论了这种异质性可能与抗原受体亲和力相关的可能性。
