Wikman-Coffelt J, Coffelt R J
Res Commun Chem Pathol Pharmacol. 1981 Feb;31(2):299-311.
Protein purification can be improved by using high acceleration - deceleraton centrifugation. This study describes a high acceleration programmable centrifuge which reaches 5,000 x g in 3 sec and brakes from 5,000 to 0 x g in 4.3 sec. This study further describes the use of this centrifuge in myosin purification and thus demonstrates that protein purification can be improved by separating particles with a high acceleration - deceleration centrifuge for the following reasons: (1) biological and chemical equilibria are immediately terminated, (2) proteolysis is reduced, (3) working time is decreased, and (4) the native state of labile proteins are better preserved. Rapid acceleration and deceleration is advantageous in reducing centrifugation time for separation of particles because it decreases diffusion time of particles and non-desirable interactions.
使用高加速-减速离心可提高蛋白质纯化效果。本研究描述了一种高加速可编程离心机，该离心机在3秒内可达到5000×g，在4.3秒内从5000×g制动到0×g。本研究进一步描述了该离心机在肌球蛋白纯化中的应用，从而证明使用高加速-减速离心机分离颗粒可提高蛋白质纯化效果，原因如下：（1）生物和化学平衡立即终止；（2）蛋白水解减少；（3）工作时间缩短；（4）不稳定蛋白质的天然状态得到更好的保存。快速加速和减速有利于减少颗粒分离的离心时间，因为它减少了颗粒的扩散时间和不良相互作用。
