Mogilevich S E, Velikiĭ N N, Khalmurodov A G
Biokhimiia. 1981 Jan;46(1):103-9.
The activity of some NAD- and NADP-dependent dehydrogenases involved in generation of the reducing equivalents for lipogenesis and the activity and some kinetic parameters of ATP-citrate (pro-3S)-lyase from rat liver, i. e. the enzyme involved in the formation of CoASAc, the primary substrate of fatty acid biosynthesis, were studied. The changes in the activity of NADP-dependent dehydrogenase and ATP-citrate(pro-3S)-lyase, as well as the affinity of the latter for sitrate and CoA and the rate of lipogenesis in starved rats and in rats kept on a carbohydrate-rich diet after starvation appeared to be parallel. Nicotinamide decreased the activity of all NADP-dependent dehydrogenases under study, which was especially well-pronounced after nicotinamide addition against increased lipogenesis. The affinity of ATP-citrate(pro-3S)-lyase for citrate and CoA decreased simultaneously with the decrease in the concentration of the latter. These changes can possibly induce the decrease of lipogenesis rate in rat liver after addition of nicotinamide.
研究了参与脂肪生成还原当量生成的一些NAD和NADP依赖性脱氢酶的活性，以及大鼠肝脏中ATP - 柠檬酸（pro - 3S）裂解酶的活性和一些动力学参数，即参与脂肪酸生物合成主要底物CoASAc形成的酶。饥饿大鼠以及饥饿后喂食富含碳水化合物饮食的大鼠中，NADP依赖性脱氢酶和ATP - 柠檬酸（pro - 3S）裂解酶的活性变化，以及后者对柠檬酸和辅酶A的亲和力和脂肪生成速率似乎是平行的。烟酰胺降低了所研究的所有NADP依赖性脱氢酶的活性，在烟酰胺添加后对抗脂肪生成增加时这一点尤为明显。ATP - 柠檬酸（pro - 3S）裂解酶对柠檬酸和辅酶A的亲和力随着后者浓度的降低而同时降低。这些变化可能导致添加烟酰胺后大鼠肝脏脂肪生成速率下降。
