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绵羊催乳素：通过荧光偏振法研究纤溶酶两个片段非共价相互作用形成的重组分子的平衡特性

Ovine prolactin: equilibrium characteristics of the recombinant molecule formed by noncovalent interaction of two fibrinolysin fragments by fluorescence polarization.

作者信息

Jibson M D, Li C H, Glaser C B

出版信息

Proc Natl Acad Sci U S A. 1981 May;78(5):2830-2. doi: 10.1073/pnas.78.5.2830.

DOI:10.1073/pnas.78.5.2830
PMID:6454891
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC319451/
Abstract

The equilibrium characteristics of the recombined molecule formed by noncovalent interaction of two fibrinolysin fragments of ovine prolactin (oPRL) have been studied with fluorescence polarization. Fluorescein isothiocyanate (isomer I) was used to label oPRL-(1-53), creating a fluorescent peptide indistinguishable from the unlabeled fragment in the complementation reaction with oPRL-(54-199). The dissociation constant of the recombinant prolactin was 0.144 microM at 30 degrees C, with a free energy of dissociation of 9.50 kcal/mol.

摘要

通过荧光偏振研究了绵羊催乳素（oPRL）的两个纤溶酶片段非共价相互作用形成的重组分子的平衡特性。用异硫氰酸荧光素（异构体I）标记oPRL-(1-53)，在与oPRL-(54-199)的互补反应中产生一种与未标记片段无法区分的荧光肽。重组催乳素在30℃时的解离常数为0.144微摩尔，解离自由能为9.50千卡/摩尔。

相似文献

1
Ovine prolactin: equilibrium characteristics of the recombinant molecule formed by noncovalent interaction of two fibrinolysin fragments by fluorescence polarization.绵羊催乳素：通过荧光偏振法研究纤溶酶两个片段非共价相互作用形成的重组分子的平衡特性
Proc Natl Acad Sci U S A. 1981 May;78(5):2830-2. doi: 10.1073/pnas.78.5.2830.
2
Radioimmunoreactivity and receptor-binding activity of the recombined molecule obtained by complementation of two fibrinolysin fragments of ovine prolactin.通过羊催乳素的两个纤溶酶片段互补获得的重组分子的放射免疫反应性和受体结合活性。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):88-90. doi: 10.1073/pnas.78.1.88.
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The fluorescein-mediated interaction of bovine serum albumin with fluorescent derivatives of prolactin and other polypeptides in polarization of fluorescence based assays.
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本文引用的文献

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APPLICATION OF FLUORESCENCE POLARIZATION TO THE ANTIGEN-ANTIBODY REACTION. THEORY AND EXPERIMENTAL METHOD.荧光偏振在抗原-抗体反应中的应用。理论与实验方法。
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Fluorescence polarization assay of plasmin, plasminogen, and plasminogen activator.
Anal Biochem. 1980 May 1;104(1):15-22. doi: 10.1016/0003-2697(80)90270-5.
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Radioimmunoreactivity and receptor-binding activity of the recombined molecule obtained by complementation of two fibrinolysin fragments of ovine prolactin.通过羊催乳素的两个纤溶酶片段互补获得的重组分子的放射免疫反应性和受体结合活性。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):88-90. doi: 10.1073/pnas.78.1.88.
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Reaction of fluorescein-isothiocyanate with proteins and amino acids. II. Preparation of fluorescein-thiohydantoin amino acids and their thin-layer chromatography.异硫氰酸荧光素与蛋白质和氨基酸的反应。II. 荧光硫代乙内酰脲氨基酸的制备及其薄层层析
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Studies on pituitary lactogenic hormone. The primary structure of the porcine hormone.垂体催乳激素的研究。猪催乳激素的一级结构。
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Assay of proteolytic enzymes by the fluorescence polarization technique.
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Two fragments from fibrinolysin digests of ovine prolactin: characterization and recombination to generate full immunoreactivity.绵羊催乳素纤维蛋白溶酶消化产生的两个片段：特性鉴定及重组以产生完全免疫反应性
Proc Natl Acad Sci U S A. 1978 May;75(5):2155-9. doi: 10.1073/pnas.75.5.2155.