• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

绵羊催乳素纤维蛋白溶酶消化产生的两个片段：特性鉴定及重组以产生完全免疫反应性

Two fragments from fibrinolysin digests of ovine prolactin: characterization and recombination to generate full immunoreactivity.

作者信息

Birk Y, Li C H

出版信息

Proc Natl Acad Sci U S A. 1978 May;75(5):2155-9. doi: 10.1073/pnas.75.5.2155.

DOI:10.1073/pnas.75.5.2155
PMID:149984
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392510/
Abstract

The action of fibrinolysin (plasmin; EC 3.4.21.7) on ovine prolactin has been investigated. It was found that the enzyme selectively cleaves the bond between Met-53 and Ala-54. The two fragments, PRL-(1-53) and PRL-(54-199), have been purified and characterized. A recombinant molecule has been obtained by noncovalent interaction of PRL-(1-53) and PRL-(54-199). The recombined protein behaves nearly identically to the parent hormone in circular dichroism spectra and exclusion chromatography. The recombinant possesses full immunoreactivity, as revealed by gel double-diffusion and complement fixation. However, the recombined protein exhibits low prolactin activity in the pigeon crop-sac test.

摘要

已对纤维蛋白溶酶（纤溶酶；EC 3.4.21.7）对绵羊催乳素的作用进行了研究。发现该酶选择性地切割甲硫氨酸-53和丙氨酸-54之间的肽键。已纯化并鉴定了两个片段，即PRL-(1-53)和PRL-(54-199)。通过PRL-(1-53)和PRL-(54-199)的非共价相互作用获得了重组分子。重组蛋白在圆二色光谱和排阻色谱中表现与亲本激素几乎相同。如凝胶双向扩散和补体结合所示，重组体具有完全的免疫反应性。然而，重组蛋白在鸽嗉囊试验中表现出低催乳素活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bef2/392510/fab7f57ebb11/pnas00017-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bef2/392510/980e78734718/pnas00017-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bef2/392510/5d3e1748a8de/pnas00017-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bef2/392510/fab7f57ebb11/pnas00017-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bef2/392510/980e78734718/pnas00017-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bef2/392510/5d3e1748a8de/pnas00017-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bef2/392510/fab7f57ebb11/pnas00017-0110-a.jpg

相似文献

1
Two fragments from fibrinolysin digests of ovine prolactin: characterization and recombination to generate full immunoreactivity.绵羊催乳素纤维蛋白溶酶消化产生的两个片段：特性鉴定及重组以产生完全免疫反应性
Proc Natl Acad Sci U S A. 1978 May;75(5):2155-9. doi: 10.1073/pnas.75.5.2155.
2
Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.人生长激素：通过激素两个片段的非共价相互作用恢复完全生物活性。
Proc Natl Acad Sci U S A. 1976 May;73(5):1476-9. doi: 10.1073/pnas.73.5.1476.
3
Radioimmunoreactivity and receptor-binding activity of the recombined molecule obtained by complementation of two fibrinolysin fragments of ovine prolactin.通过羊催乳素的两个纤溶酶片段互补获得的重组分子的放射免疫反应性和受体结合活性。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):88-90. doi: 10.1073/pnas.78.1.88.
4
Ovine prolactin: equilibrium characteristics of the recombinant molecule formed by noncovalent interaction of two fibrinolysin fragments by fluorescence polarization.绵羊催乳素：通过荧光偏振法研究纤溶酶两个片段非共价相互作用形成的重组分子的平衡特性
Proc Natl Acad Sci U S A. 1981 May;78(5):2830-2. doi: 10.1073/pnas.78.5.2830.
5
Studies on prolactin. Selective reduction of the disulfide bonds of the ovine hormone.催乳素研究。绵羊激素中二硫键的选择性还原。
Biochemistry. 1979 Oct 30;18(22):4851-60. doi: 10.1021/bi00589a013.
6
Human pituitary growth hormone: isolation and properties of two biologically active fragments from plasmin digests.人垂体生长激素：纤溶酶消化产物中两个生物活性片段的分离与特性
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1197-201. doi: 10.1073/pnas.71.4.1197.
7
Isolation and properties of two fragments from plasmin digests of human choriomammotropin.人绒毛膜促乳腺素纤溶酶消化产物中两个片段的分离与特性
Int J Pept Protein Res. 1978 Aug;12(2):114-20. doi: 10.1111/j.1399-3011.1978.tb02874.x.
8
Reaction of ovine prolactin with 2-(2-nitrophenylsulfenyl)-3-methyl-3'-bromoindolemine.绵羊催乳素与2-(2-硝基苯基亚磺酰基)-3-甲基-3'-溴吲哚胺的反应
Int J Pept Protein Res. 1978 May;11(5):345-52.
9
Studies on pituitary prolactin. 39. Reaction of the ovine hormone with hydrogen peroxide.垂体催乳素研究。39. 绵羊激素与过氧化氢的反应。
Biochim Biophys Acta. 1976 Jul 19;439(1):240-9. doi: 10.1016/0005-2795(76)90179-3.
10
Pituitary lactogenic hormone. XXXVII. Synthesis and properties of the carboxyl terminal cyclic undecapeptide.垂体催乳激素。三十七。羧基末端环十一肽的合成与性质。
Int J Pept Protein Res. 1975;7(5):389-93.

引用本文的文献

1
Ovine prolactin: equilibrium characteristics of the recombinant molecule formed by noncovalent interaction of two fibrinolysin fragments by fluorescence polarization.绵羊催乳素：通过荧光偏振法研究纤溶酶两个片段非共价相互作用形成的重组分子的平衡特性
Proc Natl Acad Sci U S A. 1981 May;78(5):2830-2. doi: 10.1073/pnas.78.5.2830.
2
Radioimmunoreactivity and receptor-binding activity of the recombined molecule obtained by complementation of two fibrinolysin fragments of ovine prolactin.通过羊催乳素的两个纤溶酶片段互补获得的重组分子的放射免疫反应性和受体结合活性。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):88-90. doi: 10.1073/pnas.78.1.88.
3

本文引用的文献

1
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition.定量微量补体结合及其在通过特异性抗原-抗体抑制研究抗原结构中的应用。
J Immunol. 1961 Sep;87:290-5.
3
Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion.凝胶中的抗原-抗体反应。IV. 扩散协调系统中的反应类型。
A single domain of human prostatic acid phosphatase shows antibody-mediated restoration of catalytic activity.
人前列腺酸性磷酸酶的单个结构域表现出抗体介导的催化活性恢复。
Proc Natl Acad Sci U S A. 1982 Oct;79(19):6052-5. doi: 10.1073/pnas.79.19.6052.
Acta Pathol Microbiol Scand. 1953;32(2):230-40.
4
Separation of dansyl-amino acids by polyamide layer chromatography.用聚酰胺薄层层析法分离丹磺酰氨基酸。
Biochim Biophys Acta. 1967 Feb 21;133(2):369-70. doi: 10.1016/0005-2795(67)90078-5.
5
Bio-assay of prolactin. Analysis of the pigeon crop-sac response to local prolactin injection by an objective and quantitative method.催乳素的生物测定。用客观定量方法分析鸽子嗉囊对局部注射催乳素的反应。
Endocrinology. 1967 Apr;80(4):641-55. doi: 10.1210/endo-80-4-641.
6
Studies on pituitary lactogenic hormone. XXX. The primary structure of the sheep hormone.垂体催乳激素研究。XXX. 绵羊激素的一级结构。
Arch Biochem Biophys. 1970 Dec;141(2):705-37. doi: 10.1016/0003-9861(70)90191-8.
7
Comparative studies of the single tryptophan residue in human chorionic somatomammotropin and human pituitary growth hormone.人绒毛膜生长催乳素和人垂体生长激素中单个色氨酸残基的比较研究。
Biochemistry. 1972 Oct 24;11(22):4179-87. doi: 10.1021/bi00772a023.
8
Circular dichroism studies on human pituitary growth hormone and ovine pituitary lactogenic hormone.关于人类垂体生长激素和绵羊垂体催乳激素的圆二色性研究。
Biochemistry. 1972 Feb 29;11(5):884-8. doi: 10.1021/bi00755a030.
9
Studies on pituitary lactogenic hormone. The primary structure of the porcine hormone.垂体催乳激素的研究。猪催乳激素的一级结构。
Int J Pept Protein Res. 1976;8(2):205-24. doi: 10.1111/j.1399-3011.1976.tb02497.x.
10
Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.人生长激素：通过激素的天然片段与合成片段的非共价相互作用恢复完全生物活性。
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1016-9. doi: 10.1073/pnas.74.3.1016.