Gardner G F, Feldman J F
Genetics. 1980 Dec;96(4):877-86. doi: 10.1093/genetics/96.4.877.
Four new circadian clock mutants of Neurospora crassa have been isolated that alter the period length of the circadian conidiation rhythm. Three of these are at the frq locus on linkage group VIIR, where four other clock mutants are located. In contrast to wild type, which has a period length of 21.6 hr, frq-6 has a period length of 19 hr, while frq-7 and frq-8 have period lengths of 29 hr and represent the largest effects of any single gene mutants on circadian periodicity. Thus, seven mutants have now been isolated that map to the frq locus, with period lengths ranging from 16.5 to 29 hr, and each mutant alters clock periodicity by an integral multiple of 2.5 hr. In addition, all frq mutants show incomplete dominance in heterokaryons. The large percentage of clock mutants that map to this locus, coupled with their unique properties, suggests that the frq locus plays an important role in clock organization.--The fourth mutant, designated chrono (chr), has a period length of 23.5 hr, shows incomplete dominance and is unlinked to either of the previously identified clock loci, frq or prd (formerly called frq-5). Double mutants between various combinations of clock mutants show additive effects and indicate no significant gene interaction among mutants at these three loci.
已分离出四种新的粗糙脉孢菌生物钟突变体，它们改变了生物钟产孢节律的周期长度。其中三个位于VIIR连锁群上的frq基因座，另外四个生物钟突变体也位于该基因座。与周期长度为21.6小时的野生型相比，frq-6的周期长度为19小时，而frq-7和frq-8的周期长度为29小时，代表了任何单个基因突变体对生物钟周期性的最大影响。因此，现已分离出七个定位于frq基因座的突变体，其周期长度在16.5至29小时之间，每个突变体将生物钟周期改变2.5小时的整数倍。此外，所有frq突变体在异核体中均表现出不完全显性。大量定位于该基因座的生物钟突变体及其独特特性表明，frq基因座在生物钟组织中起重要作用。——第四个突变体命名为chrono（chr），其周期长度为23.5小时，表现出不完全显性，并且与先前鉴定的两个生物钟基因座frq或prd（以前称为frq-5）均不连锁。生物钟突变体各种组合之间的双突变体显示出累加效应，表明这三个基因座的突变体之间没有明显的基因相互作用。
