促肿瘤佛波酯和狼毒素对人混合淋巴细胞反应的抑制作用。

Inhibitory effect of tumor promoting phorbol esters and mezerein on human mixed lymphocyte reaction.

作者信息

Yamasaki H, Martel N

出版信息

Cancer Lett. 1981 Mar;12(1-2):43-52. doi: 10.1016/0304-3835(81)90036-7.

Abstract

The effect of phorbol ester-type tumor promoters on the mixed reaction of human lymphocytes was investigated. When a low concentration (10 ng/ml, 1.67 X 10(-8) M) of 12-O-tetradecanoylphorbol-13-acetate (TPA) was added at the beginning of the mixed lymphocyte reaction, this, as measured by [3H]thymidine incorporation, was almost completely inhibited. The inhibitory effect is apparently not related to a toxic effect, since [3H]uridine incorporation was affected to a lesser extent by the addition of TPA. TPA is also inhibitory when added after the reaction has started. The inhibition of the reaction by TPA is very rapid and complete inhibition can be seen within 2 h after TPA addition. Another promoting phorbol ester, phorbol-12,13-didecanoate )PDD), also inhibited the reaction, whereas inactive derivatives, phorbol and 4 alpha-PDD, did not. Mezerein, a weak promoter on mouse skin, was also a potent inhibitor of the human mixed lymphocyte reaction.

摘要

研究了佛波酯型肿瘤启动子对人淋巴细胞混合反应的影响。当在混合淋巴细胞反应开始时加入低浓度（10 ng/ml，1.67×10⁻⁸ M）的12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）时，通过[³H]胸腺嘧啶核苷掺入法测定，反应几乎被完全抑制。这种抑制作用显然与毒性作用无关，因为加入TPA后，[³H]尿苷掺入受到的影响较小。反应开始后加入TPA也具有抑制作用。TPA对反应的抑制非常迅速，在加入TPA后2小时内即可观察到完全抑制。另一种促进性佛波酯，佛波醇 - 12,13 - 二癸酸酯（PDD），也抑制该反应，而无活性的衍生物，佛波醇和4α - PDD则没有这种作用。对小鼠皮肤有微弱促癌作用的大戟二萜醇酯，也是人混合淋巴细胞反应的有效抑制剂。