Rupe C O, Fetter M C
Clin Chem. 1981 Aug;27(8):1385-7.
In this quantitative assay, urinary urobilinogen is oxidized to urobilin with iodate in an acid medium, the pH is adjusted to 6 with sodium acetate, and the mixture is reacted with alcoholic HgCl2 solution, extracted with CHCl3, the measured spectrophotometrically at 513 nm. The artificial standards of previous methods have been replaced with crystalline stercobilin IX (commercially available), a urobilin closely related to the urinary urobilins. The reproducibility of the method, as assessed from 10 replicates of a single urine specimen to which urobilinogen was added, gave a coefficient of variation of 3.9%. Analytical recovery of urobilinogen added to urines was 90.4% (SD 14.5%). Bilirubin, biliverdin, mesobilirubin, coproporphyrin I, uroporphyrin I, and porphobilinogen do not interfere.
在该定量分析中，尿胆原在酸性介质中被碘酸盐氧化为尿胆素，用醋酸钠将pH调至6，然后该混合物与乙醇氯化汞溶液反应，用三氯甲烷萃取，在513nm处进行分光光度测定。以前方法的人工标准品已被结晶粪胆素IX（市售）取代，粪胆素IX是一种与尿胆素密切相关的尿胆素。从添加了尿胆原的单个尿液标本重复检测10次评估该方法的重现性，变异系数为3.9%。添加到尿液中的尿胆原的分析回收率为90.4%（标准差14.5%）。胆红素、胆绿素、中胆红素、粪卟啉I、尿卟啉I和卟胆原不产生干扰。
