Fetter M C, Rupe C O
Am J Med Technol. 1981 Sep;47(9):729-35.
A strip test based on Ehrlich's reaction has improved specificity for urobilinogen in urine. Strip reactions are calibrated in mg/dl using a recently developed reference assay which is based on a mercuric chloride reaction and includes a crystalline reference standard. Strip reactions correlate well with the mercuric assay when the urine sample contains only urobilinogen. If a urine contains urobilin in addition to urobilinogen, the strip test gives slightly lower values than the reference assay. The strip test reacts only with urobilinogen but the reference assay measures urobilin as well as urobilinogen. Comparative data with both tests are included for normal and pathological urines. Reaction colors with the strip test have been characterized in L* a b* values, an international basis for color designation.
一种基于埃利希反应的试纸条检测方法提高了尿液中尿胆原检测的特异性。试纸条反应采用最近开发的基于氯化汞反应并包含结晶参考标准品的参考检测方法以毫克/分升为单位进行校准。当尿液样本仅含尿胆原时，试纸条反应与汞检测法相关性良好。如果尿液中除尿胆原外还含有尿胆素，试纸条检测结果会比参考检测法略低。试纸条检测仅与尿胆原发生反应，而参考检测法同时检测尿胆素和尿胆原。文中列出了正常尿液和病理尿液两种检测方法的对比数据。试纸条检测的反应颜色已用L* a b值进行了表征，L a b*值是颜色指定的国际标准。
