Alvarez J M, de Landazuri M O, Bonnard G D, Herberman R B
J Immunol. 1978 Oct;121(4):1270-5.
Normal human T cells grown in continued cultures in medium containing conditioned medium (CM) from PHA-stimulated lymphocytes were studied for their ability to manifest three known forms of cell-mediated cytotoxicity: lectin-induced cellular cytotoxicity (LICC), natural killer cell (NK) activity, and antibody-dependent cellular cytotoxicity (ADCC). The cultured T cells (CTC) were very effective mediators of LICC, being cytotoxic even at very low attacker-target cell ratios in the presence of different lectins, and against different types of targets. When tested without the addition of lectin, the CTC demonstrated a low degree of spontaneous cytotoxicity. This spontaneous cytotoxicity might not be due to conventional NK cells however, since the CTC failed to show significant numbers of cells with Fc receptors (FcR) for IgG, and had no detectable ADCC activity. CTC could represent a population enriched in polyclonal activated T cells with low spontaneous cytotoxicity against a variety of allogeneic target cells, which is greatly enhanced by the addition of lectins dur ing the 51Cr release assay.
对在含有来自PHA刺激淋巴细胞的条件培养基（CM）的培养基中持续培养的正常人T细胞进行研究，以观察它们表现出三种已知形式的细胞介导细胞毒性的能力：凝集素诱导的细胞毒性（LICC）、自然杀伤细胞（NK）活性和抗体依赖性细胞毒性（ADCC）。培养的T细胞（CTC）是LICC的非常有效的介质，即使在存在不同凝集素且攻击细胞与靶细胞比例非常低的情况下，对不同类型的靶细胞也具有细胞毒性。在不添加凝集素的情况下进行测试时，CTC表现出低度的自发细胞毒性。然而，这种自发细胞毒性可能不是由于传统的NK细胞，因为CTC未能显示出大量具有IgG Fc受体（FcR）的细胞，并且没有可检测到的ADCC活性。CTC可能代表了一群富含多克隆活化T细胞的群体，对各种同种异体靶细胞具有低自发细胞毒性，在51Cr释放试验期间添加凝集素可大大增强这种毒性。
