粗糙脉孢菌热处理大分生孢子中核仁周围致密斑点的超微结构细胞化学
Ultrastructural cytochemistry of perinucleolar dense spots in heat-treated macroconidia of Neurospora crassa.
作者信息
Ton-That T C, Turian G, Fakan J, Gautier A
出版信息
Eur J Cell Biol. 1981 Jun;24(2):317-9.
PMID:6456905
Abstract
Upon heat treatment at 46 degrees C of Neurospora macroconidia, numerous dense sports appear around the nucleolus but disappear completely 5 h after shift-down to normal temperature. Cytochemical reactions and high-resolution autoradiography after pulse-labeling with tritiated uridine indicate that these dense sports contain RNA (perhaps pre-rRNA), RNP and other proteins, but no DNA.
摘要
将粗糙脉孢菌大分生孢子在46摄氏度下进行热处理后，核仁周围会出现许多致密小体，但在转移到正常温度5小时后会完全消失。用氚标记的尿苷进行脉冲标记后的细胞化学反应和高分辨率放射自显影表明，这些致密小体含有RNA（可能是前体核糖体RNA）、核糖核蛋白和其他蛋白质，但不含DNA。
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