Machwe A, Kapoor M
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Biochem Biophys Res Commun. 1993 Oct 29;196(2):692-8. doi: 10.1006/bbrc.1993.2305.
Heat shock and other stress treatments, resulting in thermotolerance in Neurospora crassa cells, stimulate the induction of a peroxidase at a high level. The putative gene encoding this heat shock-induced peroxidase (hspp) has been cloned, using a cDNA clone of the manganese peroxidase of Phanerochaete chrysosporium, as a probe. Northern blot analysis of total RNA from heat-shocked cell showed the stress-dependent accumulation of a approximately 10 kb transcript. The identity of the HSP, corresponding to the heat-induced peroxidase, was established using hybrid-arrested in vitro translation.
热休克及其他应激处理可使粗糙脉孢菌细胞产生耐热性，并刺激高水平过氧化物酶的诱导。利用黄孢原毛平革菌锰过氧化物酶的cDNA克隆作为探针，已克隆出编码这种热休克诱导过氧化物酶（hspp）的推定基因。对热休克细胞的总RNA进行Northern印迹分析，结果显示一种约10 kb转录本的应激依赖性积累。利用杂交捕获体外翻译确定了与热诱导过氧化物酶相对应的热休克蛋白（HSP）的身份。
