Saito T, Osono T, Inoue M, Mitsuhashi S
J Gen Virol. 1981 Aug;55(Pt 2):451-7. doi: 10.1099/0022-1317-55-2-451.
Three kinds of temperate Staphylococcus aureus phages were differentiated by serological, sedimentation and electron microscopic studies. Phage S1 had a long hexagonal head and a long tail, a density in Cs2SO4 of 1.364 g/ml and belonged to serological group A; phage S2 had a short hexagonal head and a short tail, a density of 1.385 to 1.392 g/ml and belonged to serological group B; phage S3 had a shape similar to phage S2 except for some minor differences, but had a density of 1.416 g/ml and belonged to serological group F. S. aureus MS27 was found to be singly lysogenic for S2. However, S. aureus MS3878 was a doubly lysogenic strain carrying S1 and S2 and S. aureus E169 was a triply lysogenic strain carrying S1, S2 and S3. All these temperate phages showed similarity in shape to typing phages belonging to the same serological group. The temperate S. aureus phages revealed the presence of a hexagonal baseplate with spikes. The burst sizes of phages of S1, S2 and S3 were about 50, 110 and 120 respectively. The DNA from S1, S2 and S3 ranged from 29.4 to 30 megadaltons in size.
通过血清学、沉降和电子显微镜研究区分了三种温和型金黄色葡萄球菌噬菌体。噬菌体S1具有长六边形头部和长尾部，在Cs2SO4中的密度为1.364 g/ml，属于血清学A组；噬菌体S2具有短六边形头部和短尾部，密度为1.385至1.392 g/ml，属于血清学B组；噬菌体S3除了一些细微差异外，形状与噬菌体S2相似，但密度为1.416 g/ml，属于血清学F组。发现金黄色葡萄球菌MS27对S2是单溶原性的。然而，金黄色葡萄球菌MS3878是携带S1和S2的双溶原性菌株，金黄色葡萄球菌E169是携带S1、S2和S3的三溶原性菌株。所有这些温和型噬菌体在形状上与属于同一血清学组的分型噬菌体相似。温和型金黄色葡萄球菌噬菌体显示出带有刺突的六边形基板。噬菌体S1、S2和S3的裂解量分别约为50、110和120。来自S1、S2和S3的DNA大小在29.4至30兆道尔顿之间。
