Department of Pediatrics, Kochi Medical School, Nankoku, Kochi 783-8505, Japan.
Arch Virol. 2010 Apr;155(4):545-52. doi: 10.1007/s00705-010-0623-2. Epub 2010 Mar 12.
A novel bacteriophage, phiMR25, was isolated from a lysogenic Staphylococcus aureus strain by mitomycin C induction. Its biological features were analyzed in comparison with phiMR11, which was described previously as a prototype therapeutic phage. phiMR25 is morphologically similar to phiMR11 (morphotype B1 of family Myoviridae) but has a broader host range than phiMR11 on S. aureus strains. phiMR25 can also multiply on S. aureus lysogens of phiMR11. Its DNA is 44,342 bp in size, is predicted to include 70 open reading frames, and does not contain genes related to toxin or drug resistance. The lysogenic module and most of the putative virion protein genes are completely different from those of phiMR11. In spite of their genetic diversity, intraperitoneal administration of phiMR25 rescued mice inoculated with a lethal dose of S. aureus, as was the case for phiMR11. These results suggest that phiMR25 could be another candidate phage to treat S. aureus infection.
一株新型噬菌体 phiMR25 通过丝裂霉素 C 诱导从溶原性金黄色葡萄球菌菌株中分离得到。将其生物学特征与先前描述为治疗性噬菌体原型的 phiMR11 进行比较分析。phiMR25 与 phiMR11 在形态上相似（肌尾噬菌体科 B1 形态），但在金黄色葡萄球菌菌株上的宿主范围比 phiMR11 更广。phiMR25 还可以在 phiMR11 的金黄色葡萄球菌溶原菌上增殖。其 DNA 大小为 44342bp，预计包含 70 个开放阅读框，不包含与毒素或耐药性相关的基因。溶原模块和大多数假定的病毒蛋白基因与 phiMR11 完全不同。尽管存在遗传多样性，但 phiMR25 的腹腔给药可挽救接种致死剂量金黄色葡萄球菌的小鼠，这与 phiMR11 的情况相同。这些结果表明，phiMR25 可能是另一种治疗金黄色葡萄球菌感染的候选噬菌体。
