Amann E P, Reeve J N
Mol Gen Genet. 1981;182(2):299-303. doi: 10.1007/BF00269674.
In the preceding paper (Amann et al. 1981) we described the in vitro construction of hybrids between Escherichia coli phage lambda NM607 imm434 and B. subtilis phage SPP1. These lambda/SPP1 hybrids have been used to infect minicells produced by E. coli strain DS410. Analysis on polyacrylamide gels of 35S-methionine labeled proteins synthesized in infected minicells revealed the expression of both lambda and SPP1 genes. Infection of E. coli minicells carrying plasmid pGY101, which encodes and expresses the repressor gene of phage 434, results in the selective expression of the cloned SPP1 DNA. This has resulted in the assignment of 26 out of a total of 46 known SPP1 polypeptides (Mertens et al. 1979) to individual SPP1 DNA fragments. In addition, several lambda/SPP1 fusion peptides whose transcription either originates from lambda promoters or from promoters located on the inserted SPP1 fragment, were identified.
在前一篇论文（阿曼等人，1981年）中，我们描述了大肠杆菌噬菌体λNM607 imm434与枯草芽孢杆菌噬菌体SPP1之间体外杂种的构建。这些λ/SPP1杂种已被用于感染大肠杆菌菌株DS410产生的微细胞。对感染的微细胞中合成的35S-甲硫氨酸标记蛋白进行聚丙烯酰胺凝胶分析，揭示了λ和SPP1基因的表达。感染携带质粒pGY101的大肠杆菌微细胞，该质粒编码并表达噬菌体434的阻遏基因，导致克隆的SPP1 DNA的选择性表达。这使得总共46种已知的SPP1多肽（默滕斯等人，1979年）中的26种被分配到单个SPP1 DNA片段。此外，还鉴定了几种λ/SPP1融合肽，其转录要么起源于λ启动子，要么起源于插入的SPP1片段上的启动子。
