[Isolation of various fractions of human placental nuclei and their utilization in an in vitro transcription system].
作者信息
Lasbennes A, Hess K, Ramboarina L, Belleville F, Nabet P, Paysant P
出版信息
C R Seances Soc Biol Fil. 1981;175(4):508-16.
PMID:6457675
Abstract
A new approach to isolating chromatin and DNA from human placental nuclei is described. Criteria of purity are given by spectrophotometry and chemical analysis. Chromatin and DNA keep their template efficiencies after isolation. We used them in a transcription system where wheat germ RNA polymerase intervenes. The role of histones was tested on this system.
摘要
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