体外培养的人淋巴细胞中卤代烷引起的DNA损伤。

DNA damage by haloalkanes in human lymphocytes cultured in vitro.

作者信息

Perocco P, Prodi G

出版信息

Cancer Lett. 1981 Aug;13(3):213-8. doi: 10.1016/0304-3835(81)90020-3.

Abstract

The DNA damaging activity of 7 haloalkanes was studied in a short-term in vitro system which utilized human lymphocytes. The parameters studied were the inhibition of scheduled (duplicative) and unscheduled (reparative) DNA syntheses seen as tritiated thymidine uptake. The results obtained suggested that chloromethyl methyl ether (CMME), 1,2-dibromoethane (DBE), trichloroethylene (TCE) and 1,2-dichloroethane (DCE) gave positive results such as DNA damaging agents, while carbon tetrachloride (CTC), chloroform (TCM) and dichloromethane (DCM) gave low or negative results.

摘要

在一个利用人类淋巴细胞的短期体外系统中研究了7种卤代烷的DNA损伤活性。所研究的参数是作为氚化胸腺嘧啶核苷摄取量所观察到的定时（复制性）和不定时（修复性）DNA合成的抑制情况。所得结果表明，氯甲基甲醚（CMME）、1,2 - 二溴乙烷（DBE）、三氯乙烯（TCE）和1,2 - 二氯乙烷（DCE）作为DNA损伤剂给出了阳性结果，而四氯化碳（CTC）、氯仿（TCM）和二氯甲烷（DCM）给出了低或阴性结果。