Berrih S, Gaud C, Bach M A, Le Brigand H, Binet J P, Bach J F
Clin Exp Immunol. 1981 Jul;45(1):1-8.
Functional T cell subsets have been evaluated in the peripheral blood of patients with myasthenia gravis using monoclonal anti-T cell antibodies and a suppressor cell assay based on the suppression of the mixed-lymphocyte reaction by concanavalin A-activated lymphocytes. A significant decline of suppressor cells was found in a large proportion of patients, both by direct count using the anti-suppressor-cytotoxic T cell antibody (OKT8) and by the suppressor assay. Patients also showed an increase in immature T cells defined by their simultaneous reaction with the anti-helper cell (OKT4) and anti-suppressor cell (OKT8) antibody. Thymectomy tended to enhance the deficit in suppressor cells, and to induce the disappearance of double-labelled cells.
运用单克隆抗T细胞抗体以及基于伴刀豆球蛋白A激活淋巴细胞对混合淋巴细胞反应的抑制作用的抑制细胞检测法，对重症肌无力患者外周血中的功能性T细胞亚群进行了评估。通过使用抗抑制性细胞毒性T细胞抗体（OKT8）直接计数以及抑制细胞检测法发现，大部分患者的抑制细胞显著减少。患者还表现出未成熟T细胞增多，这些未成熟T细胞通过同时与抗辅助细胞（OKT4）和抗抑制细胞（OKT8）抗体发生反应来定义。胸腺切除术往往会加剧抑制细胞的缺陷，并导致双标记细胞消失。
