使用单克隆抗体对自身免疫性疾病中的T淋巴细胞亚群进行计数。
Enumeration of T lymphocytes subsets in autoimmune disease using monoclonal antibodies.
作者信息
Raeman F, De Cock W, De Beukelaar T, De Cree J, Verhaegen H
出版信息
Clin Exp Immunol. 1981 Sep;45(3):475-9.
The numbers of T lymphocytes, B lymphocytes, helper and suppressor T lymphocytes were measured in peripheral blood of patients with autoimmune disease (rheumatoid arthritis, erythema nodosum, Sjögren's disease, Wegener's disease, idiopathic thrombocytopenia, pernicious anaemia and Hashimoto's disease). B lymphocytes were enumerated by direct immunofluorescence and T lymphocytes by E rosette tests and by indirect immunofluorescence with OKT3.PAN Helper and suppressor T lymphocytes were determined by indirect immunofluorescence with OKT4.IND and OKT8.SUP respectively. The numbers of T lymphocytes, B lymphocytes and helper T lymphocytes in patients with autoimmune disease were normal, but the numbers of suppressor T lymphocytes were significantly lower.
对患有自身免疫性疾病（类风湿性关节炎、结节性红斑、干燥综合征、韦格纳氏病、特发性血小板减少症、恶性贫血和桥本氏病）患者的外周血中的T淋巴细胞、B淋巴细胞、辅助性T淋巴细胞和抑制性T淋巴细胞数量进行了测定。B淋巴细胞通过直接免疫荧光法计数，T淋巴细胞通过E花环试验以及用OKT3进行间接免疫荧光法计数。辅助性T淋巴细胞和抑制性T淋巴细胞分别通过用OKT4.IND和OKT8.SUP进行间接免疫荧光法测定。自身免疫性疾病患者的T淋巴细胞、B淋巴细胞和辅助性T淋巴细胞数量正常，但抑制性T淋巴细胞数量显著降低。
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