Dogterom J, van Wimersma Greidanus T B, De Wied D
Am J Physiol. 1978 May;234(5):E463-7. doi: 10.1152/ajpendo.1978.234.5.E463.
Arginine-8-vasopressin (AVP) levels were measured by a sensitive and specific radioimmunoassay (RIA) in plasma and cerebrospinal fluid (CSF) of three species man, dog, and rat (Wistar and the Brattleboro strain). Basal plasma values were 1.7 pg/ml in Wistar rat, and 2.4 pg/ml in dog. Pentobarbitone, used as anesthetic during collection of CSF from dog and rat, caused a significant rise of plasma AVP values in Wistar rats, but not in dogs. After withdrawal of CSF, the plasma AVP levels of Wistar rats were increased to 29.5 +/- 9.5 pg/ml, whereas the CSF levels from the same animals were 11.5 +/- 3.9 pg/ml. The response to the various stimuli was similar in Brattleboro rats, heterozygous for hereditary hypothalamic diabetes insipidus, and in Wistar rats. In Brattleboro rats, homozygous for hereditary hypothalamic diabetes insipidus, AVP was neither detectable in plasma nor in CSF. In dog and man, AVP levels in CSF samples were higher than in simultaneously obtained plasma samples. The possibility that AVP present in CSF, might be released directly from the synthetizing hypothalamic nuclei into the ventricular system is discussed.
采用灵敏且特异的放射免疫分析法(RIA)测定了人、狗和大鼠(Wistar大鼠和Brattleboro品系)三种物种血浆和脑脊液(CSF)中的精氨酸 - 8 - 加压素(AVP)水平。Wistar大鼠的基础血浆值为1.7 pg/ml,狗为2.4 pg/ml。戊巴比妥在从狗和大鼠采集脑脊液期间用作麻醉剂,它使Wistar大鼠的血浆AVP值显著升高,但对狗没有影响。抽取脑脊液后,Wistar大鼠的血浆AVP水平升至29.5±9.5 pg/ml,而同一动物的脑脊液水平为11.5±3.9 pg/ml。遗传性下丘脑尿崩症杂合子的Brattleboro大鼠对各种刺激的反应与Wistar大鼠相似。遗传性下丘脑尿崩症纯合子的Brattleboro大鼠在血浆和脑脊液中均未检测到AVP。在狗和人身上,脑脊液样本中的AVP水平高于同时采集的血浆样本。文中讨论了脑脊液中存在的AVP可能直接从合成AVP的下丘脑核释放到脑室系统的可能性。