te Kronnie G, Donselaar Y, Soukup T, van Raamsdonk W
Histochemistry. 1981;73(1):65-74. doi: 10.1007/BF00493134.
Mammalian intrafusal fibre types (nuclear chain, nuclear bag1 and nuclear bag2 fibres) are known to differ in their ultrastructure, intensity of the myofibrillar histochemical ATP-ase reaction, type of innervation and time course of contraction. The present study concerns the myosin composition of these intrafusal fibre types in the soleus muscle (mouse) and the extensor digitorum longus muscle (rat). We used an immunohistochemical method with three myosin antisera raised in rabbits: anti chicken pectoral myosin, anti chicken heart myosin (1) and anti chicken heart myosin (2) (= anti chicken heart myosin (1) adsorbed with muscle powder from soleus muscle of guinea pig). The results showed that three intrafusal fibre types differed in their myosin composition. A comparison of intrafusal fibre types with extrafusal fibre types for the histochemical myofibrillar ATP-ase reactivity and the reactivity with myosin antisera showed a resemblance of nuclear chain fibres with extrafusal type II fibres and a difference between nuclear bag1 and nuclear bag2 fibres and all other fibre types.
哺乳动物的梭内肌纤维类型（核链纤维、核袋1纤维和核袋2纤维）在超微结构、肌原纤维组织化学ATP酶反应强度、神经支配类型和收缩时程方面存在差异。本研究关注比目鱼肌（小鼠）和趾长伸肌（大鼠）中这些梭内肌纤维类型的肌球蛋白组成。我们使用了一种免疫组织化学方法，该方法采用了三种在兔子体内产生的肌球蛋白抗血清：抗鸡胸肌肌球蛋白、抗鸡心肌肌球蛋白（1）和抗鸡心肌肌球蛋白（2）（=用豚鼠比目鱼肌肌肉粉末吸附的抗鸡心肌肌球蛋白（1））。结果表明，三种梭内肌纤维类型在肌球蛋白组成上存在差异。对梭内肌纤维类型与梭外肌纤维类型进行组织化学肌原纤维ATP酶反应性以及与肌球蛋白抗血清反应性的比较，结果显示核链纤维与梭外II型纤维相似，而核袋1纤维和核袋2纤维与所有其他纤维类型不同。
