Kucera J, Walro J M
Department of Neurology, School of Medicine, Boston University, MA 02118.
Histochemistry. 1988;90(2):151-60. doi: 10.1007/BF00500980.
Muscle spindles were either deafferented or deefferented by selectively severing the sensory or motor nerve supply to neonatal soleus muscles of rats at a time when spindles are formed but when intrafusal muscle fibers are structurally and immunocytochemically immature. Experimental muscles were excised two months after nerve section. Control and experimental spindles were examined using monoclonal antibodies specific for myosin heavy chains of slow-tonic (ALD58) and fast-twitch (MF30) chicken muscles. Only intrafusal fibers bound these antibodies in intact soleus muscles. The deefferented spindles exhibited a pattern of ALD58 and MF30 binding similar to that of normal adult intrafusal fibers, whereas deafferented intrafusal fibers were unreactive with the two antibodies. Thus intact sensory innervation is essential for myosin heavy chain expression in intrafusal muscle fibers during postnatal development of rat spindles.
在大鼠比目鱼肌梭形成但梭内肌纤维在结构和免疫细胞化学方面尚未成熟时，通过选择性切断新生大鼠比目鱼肌的感觉或运动神经供应，使肌梭去传入或去传出。神经切断两个月后切除实验肌肉。使用针对慢张力（ALD58）和快肌（MF30）鸡肌肉肌球蛋白重链的单克隆抗体检查对照和实验肌梭。在完整的比目鱼肌中，只有梭内纤维与这些抗体结合。去传出的肌梭显示出与正常成年梭内纤维相似的ALD58和MF30结合模式，而去传入的梭内纤维与这两种抗体无反应。因此，完整的感觉神经支配对于大鼠肌梭出生后发育过程中梭内肌纤维肌球蛋白重链的表达至关重要。
