Wulff J C, Held H, Mueller-Hermelink H K, Schlaak M
Clin Exp Immunol. 1981 Jun;44(3):485-90.
This study was conducted in order to clarify further the role of bone marrow lymphocyte (BML) subsets in graft-versus-host disease (GVHD) after clinical BM transplantation. Human BML separated by velocity sedimentation through a continuous sucrose gradient were found to consist of 38% T, 21% B and 41% null cells. T cells were purified from BML or peripheral blood lymphocytes (PBL) by rosette formation with sheep red blood cells (SRBC) and subsequent gradient centrifugation. Mitogenic stimulation of BML, PBL and the respective T cell subsets revealed the well-known reactivity of mature T lymphocytes, whereas non-T lymphocytes comprising T cell precursors showed only a weak response. In allogeneic stimulation kinetics, however, non-T-BML disclosed a delayed but marked responsiveness as compared to BML and T-BML suggesting that a T cell precursor matures under culture conditions. This cell type could be involved in GVHD in addition to mature T cells after clinical bone marrow transplantation.
进行本研究是为了进一步阐明临床骨髓移植后骨髓淋巴细胞（BML）亚群在移植物抗宿主病（GVHD）中的作用。通过连续蔗糖梯度速度沉降分离的人BML被发现由38%的T细胞、21%的B细胞和41%的裸细胞组成。通过与绵羊红细胞（SRBC）形成玫瑰花结并随后进行梯度离心，从BML或外周血淋巴细胞（PBL）中纯化T细胞。对BML、PBL和各自的T细胞亚群进行促有丝分裂刺激，揭示了成熟T淋巴细胞众所周知的反应性，而包含T细胞前体的非T淋巴细胞仅表现出微弱反应。然而，在同种异体刺激动力学中，与BML和T - BML相比，非T - BML显示出延迟但明显的反应性，这表明T细胞前体在培养条件下成熟。除了临床骨髓移植后的成熟T细胞外，这种细胞类型可能参与GVHD。
