Burns J B, Zweiman B, Lisak R P
J Clin Immunol. 1981 Jul;1(3):195-200. doi: 10.1007/BF00922763.
Investigations of central nervous system cellular immune reactivity in human disease, as reflected in the responses of cerebrospinal fluid lymphocytes, have been limited primarily due to the low numbers of cerebrospinal fluid lymphocytes available during routine diagnostic lumbar punctures in normal individuals and most patients with demyelinating diseases. We report the use of a T-cell growth factor generated by by phytochemagglutinin-stimulated, irradiated normal peripheral blood lymphocytes to maintain long-term proliferating cultures of human cerebrospinal fluid lymphocytes. Cerebrospinal fluid lymphocytes. Cerebrospinal fluid T-cell cultures were initiated from 10 to 14 cerebrospinal fluid samples with up to 5000-fold expansion of initial cell numbers. Few, if any, macrophage or surface immunoglobulin-bearing cells were present, while 80 to 90% of the cultured cells were T cells as demonstrated by rosette formation with sheep red blood cells. Mixed lymphocyte cultures with cultured cerebrospinal fluid T cells and irradiated, freshly isolated allogeneic peripheral blood lymphocytes yielded a positive response in four of the five cultures tested.
对人类疾病中枢神经系统细胞免疫反应性的研究，如通过脑脊液淋巴细胞的反应所反映的那样，主要受到限制，这是因为在正常个体和大多数脱髓鞘疾病患者进行常规诊断性腰椎穿刺时，可获得的脑脊液淋巴细胞数量较少。我们报告了使用由植物血凝素刺激、照射过的正常外周血淋巴细胞产生的一种T细胞生长因子来维持人类脑脊液淋巴细胞的长期增殖培养。脑脊液淋巴细胞。从10至14份脑脊液样本开始进行脑脊液T细胞培养，初始细胞数量最多可扩增5000倍。几乎没有巨噬细胞或带有表面免疫球蛋白的细胞，而通过与绵羊红细胞形成玫瑰花结证明，80%至90%的培养细胞为T细胞。用培养的脑脊液T细胞与照射过的、新鲜分离的同种异体外周血淋巴细胞进行混合淋巴细胞培养，在所测试的5种培养物中有4种产生了阳性反应。
