Raff M C
Immunology. 1970 Oct;19(4):637-50.
Immunofluorescent staining has been used to detect antigenic determinants on the surface of living lymphoid cells of mice. It has been possible to distinguish two distinct populations of peripheral lymphocytes: one population carries the theta isoantigen and is thymus-dependent; the other has naturally-occurring immunoglobulin determinants on the cell surface and appears to be thymus-independent. The relative proportions of these two populations of cells is characteristic for each type of lymphoid tissue and these proportions have been determined for thymus, lymph node, spleen, and thoracic duct cells.
免疫荧光染色已被用于检测小鼠活淋巴细胞表面的抗原决定簇。现已能够区分外周淋巴细胞的两个不同群体：一个群体携带θ同种抗原，且依赖胸腺；另一个群体在细胞表面具有天然存在的免疫球蛋白决定簇，似乎不依赖胸腺。这两种细胞群体的相对比例对于每种淋巴组织类型而言都是独特的，并且已经确定了胸腺、淋巴结、脾脏和胸导管细胞的这些比例。
