Ahlers I, Ahlersová E, Kapuriková K, Toropila M, Smajda B
Physiol Bohemoslov. 1982;31(1):75-82.
Young male rats of a conventional Wistar breed were adapted for several weeks (3-6) to a 12:12 h light:dark (7 a.m. - 7 p.m., 7 p.m. - 7 a.m.) regimen (LD), to inversion of this standard regimen (DL), to continuous darkness (DD) or to continuous light (LL). After adaptation, groups of animals were killed at 3-hour intervals during the day and the basic lipid fractions were determined in their serum. Under the standard light regimen the cosinor test demonstrated a rhythm in all the indicators (triacylglycerols, total cholesterol, phospholipids) except for non-esterified fatty acids. When the regimen was inverted (DL), the peaks of the circadian non-esterified fatty acid and triacylglycerol curves shifted to the antiphase. The acrophases of "free-running" serum lipid rhythm under the DD regimen of the standard regimen rhythms differed in the case of cholesterol and phospholipids. In the case of triacylglycerols and phospholipids there was disagreement between the DD and LL regimen curves. With reference to the localization of the acrophase under the DD and LD regimens it was assumed that the influence of the light regimen on the synchronization of circadian rhythms is small in the case of serum non-esterified fatty acids and triacylglycerols and that it is greater in the case of serum cholesterol and phospholipids.
将常规Wistar品系的年轻雄性大鼠饲养数周（3 - 6周），使其适应12:12小时光照:黑暗（上午7点 - 下午7点，下午7点 - 上午7点）的光照周期（LD）、该标准光照周期的倒置（DL）、持续黑暗（DD）或持续光照（LL）环境。适应期过后，在白天每隔3小时处死一组动物，并测定其血清中的基本脂质成分。在标准光照周期下，除游离脂肪酸外，余弦检验表明所有指标（三酰甘油、总胆固醇、磷脂）均存在节律。当光照周期倒置（DL）时，昼夜游离脂肪酸和三酰甘油曲线的峰值移至反相。在DD光照周期下，标准光照周期节律中“自由运行”的血清脂质节律的峰相位在胆固醇和磷脂的情况下有所不同。在三酰甘油和磷脂的情况下，DD和LL光照周期曲线不一致。关于DD和LD光照周期下峰相位的定位，推测光照周期对血清游离脂肪酸和三酰甘油昼夜节律同步的影响较小，而对血清胆固醇和磷脂的影响较大。
