Shifrine M, Taylor N J, Rosenblatt L S, Wilson F D
Am J Vet Res. 1978 Apr;39(4):687-90.
The optimum mitogen concentration and time required for using whole blood from dogs in a microassay were determined, and this test then was compared with a standard lymphocyte-stimulation microtest, using gradient-isolated lymphocytes, 2 different mitogens (phytohemagglutinin and concanavalin A), and 2 different culture media. Statistical analysis of the data from 10 dogs showed that whole blood was significantly more reactive than were gradient-isolated lymphocytes (P less than 0.05). Waymouth's medium was significantly better than RPMI 1640 (P less than 0.001), and concanavalin A was significantly more mitogenic than phytohemagglutinin (P less than 0.001). The interaction between lymphocyte source and mitogens was the only one of the various interactions that was significant at P less than 0.05.
确定了在微量测定中使用犬全血所需的最佳促有丝分裂原浓度和时间，然后将该试验与标准淋巴细胞刺激微量试验进行比较，后者使用梯度分离的淋巴细胞、两种不同的促有丝分裂原（植物血凝素和刀豆球蛋白A）以及两种不同的培养基。对10只犬的数据进行的统计分析表明，全血的反应性显著高于梯度分离的淋巴细胞（P<0.05）。Waymouth培养基显著优于RPMI 1640（P<0.001），刀豆球蛋白A的促有丝分裂作用显著强于植物血凝素（P<0.001）。淋巴细胞来源与促有丝分裂原之间的相互作用是各种相互作用中唯一在P<0.05时具有显著性的。
