Mullins D W, Senaratne N, Lacey J C
Orig Life. 1984;14(1-4):597-604. doi: 10.1007/BF00933709.
In the present paper, we report on the effect of pH and carbonate on the hydrolysis rate constants of N-blocked and free aminoacyl adenylate anhydrides. Whereas the hydrolysis of free aminoacyl adenylates seems principally catalyzed by OH-, the hydrolysis of the N-blocked species is also catalyzed by H+, giving this compound a U-shaped hydrolysis vs. pH curve. Furthermore, at pH's less than 8, carbonate has an extreme catalytic effect on the hydrolysis of free aminoacyl-AMP anhydride, but essentially no effect on the hydrolysis of N-blocked aminoacyl-AMP anhydride. Furthermore, the N-blocked aminoacyl-AMP anhydride is a very efficient generator of peptides using free glycine as acceptor. The possible significance of the observations to prebiological peptide synthesis is discussed.
在本论文中，我们报道了pH值和碳酸盐对N-保护的和游离的氨酰腺苷酸酐水解速率常数的影响。游离氨酰腺苷酸的水解似乎主要由OH-催化，而N-保护物种的水解也由H+催化，使该化合物具有水解速率随pH值变化的U形曲线。此外，在pH值小于8时，碳酸盐对游离氨酰-AMP酐的水解有极大的催化作用，但对N-保护的氨酰-AMP酐的水解基本没有影响。此外，N-保护的氨酰-AMP酐是以游离甘氨酸为受体的非常有效的肽生成剂。讨论了这些观察结果对生命起源前肽合成的可能意义。
