Sakamoto H, Firkin F C, Chesterman C N
Pathology. 1984 Apr;16(2):126-30. doi: 10.3109/00313028409059089.
The influence of material released by activated human platelets on the phagocytic activity of human neutrophils and monocytes has been investigated under conditions where there was a low background level of exposure of the leukocytes to platelet release products (PRPr). Incubation with PRPr prepared from 400 X 10(9)/l platelets resulted in an approximate 3-fold increase in the proportion of leukocytes which ingested particles. Stimulation was produced by dilutions of PRPr down to 1:4 to indicate that phagocytic activity was affected by material released by concentrations of platelets within the physiological range. This response appeared to reflect stimulation of the overall phagocytic capability of the leukocytes as equivalent ingestion was observed with particles which possessed or lacked the capacity to bind complement to their surface. Enhancement of phagocytic activity was evidently secondary to induction of a change in leukocyte behaviour which was sustained in the absence of continued exposure to preparations of PRPr. These observations extend the range of human biological processes known to be responsive to PRPr, and have implications for the assessment of leukocyte phagocytic activity in vitro as this particular property could be modified by contact with PRPr during the isolation of leukocytes from peripheral blood.
在白细胞与血小板释放产物（PRPr）的背景暴露水平较低的条件下，研究了活化的人血小板释放的物质对人中性粒细胞和单核细胞吞噬活性的影响。用从400×10⁹ /升血小板制备的PRPr孵育，导致摄取颗粒的白细胞比例增加了约3倍。PRPr稀释至1:4仍能产生刺激作用，这表明生理范围内血小板浓度释放的物质会影响吞噬活性。这种反应似乎反映了白细胞整体吞噬能力的刺激，因为观察到具有或不具有表面结合补体能力的颗粒的摄取量相当。吞噬活性的增强显然是白细胞行为发生变化的结果，这种变化在不再持续接触PRPr制剂的情况下仍能持续。这些观察结果扩展了已知对PRPr有反应的人类生物学过程的范围，并且对体外白细胞吞噬活性的评估具有启示意义，因为在从外周血分离白细胞的过程中，这种特殊性质可能会因与PRPr接触而改变。
