Figueiras H, Jacobsson S O, Jones I, Luthman J
Nord Vet Med. 1984 May-Jun;36(5-6):182-8.
The glutathione peroxidase activity (GSH-Px) and the in vitro erythrocyte uptake of 75Se (ESeU) was followed in blood samples from 31 lambs for 105 days. The lambs were divided into three groups; untreated (group U), vitamin E treated (group E) and selenium treated (group Se). The animals in group E were administered intramuscularly 250 mg vitamin E at two weeks interval and those in group Se 0.1 mg Se/kg as sodium selenite monthly. None of the lambs exhibited symptoms of muscular degeneration or any other disease during the trial. Vitamin E had no effect neither on the GSH-Px nor on the ESeU. Thirty days and later after the first Se treatment the GSH-Px was significantly higher in group Se than in the other two groups, but the most rapid increase of GSH-Px in this group was noticed after 44 days. On day 51 a significant decrease of GSH-Px was shown in groups U and E compared with day O. The lambs in groups U and E had significantly higher ESeU on day 36 and later compared with day 0. The administration of selenium in the mineral supplement (10 mg Se/kg) to the lambs in group U and E during the 26 latest days of the study gave an almost significant increase of GSH-Px and a decrease of ESeU. Our experience of the ESeU-test is that it is a simple method, and can be used to evaluate Se status of animals.
对31只羔羊的血样进行了105天的谷胱甘肽过氧化物酶活性（GSH-Px）和75硒的体外红细胞摄取量（ESeU）跟踪监测。羔羊被分为三组：未处理组（U组）、维生素E处理组（E组）和硒处理组（Se组）。E组动物每隔两周肌肉注射250毫克维生素E，Se组动物每月注射0.1毫克/千克亚硒酸钠形式的硒。在试验期间，没有一只羔羊表现出肌肉退化或任何其他疾病的症状。维生素E对GSH-Px和ESeU均无影响。首次硒处理30天后及之后，Se组的GSH-Px显著高于其他两组，但该组GSH-Px在44天后增长最为迅速。与第0天相比，U组和E组在第51天GSH-Px显著下降。U组和E组的羔羊在第36天及之后的ESeU显著高于第0天。在研究的最后26天，给U组和E组的羔羊在矿物质补充剂中添加硒（10毫克/千克），使GSH-Px几乎显著增加，ESeU下降。我们对ESeU试验的经验是，它是一种简单的方法，可用于评估动物的硒状态。
