Durand R E
Cancer Res. 1975 May;35(5):1295-300.
Chinese hamster V79-171 cells grown in vitro as asynchronous single cells, as plateau-phase cultures, and as multicell spheroids contianed subpopulations that differed in cell volume. These subpopulations could be isolated according to their sedimentation velocity at unit gravity. Asynchronous cells were found to sediment at a modal velocity of 17.7 mm/hr, whereas the modal sedimentation velocities of plateau and spheroid cells were found to be 16.0 and 15.1 mm/hr, respectively. When the composition of each subpopulation was determined using either [3H]thymindine incorporation or sensitivity to ionizing radiation as an index of cell cycle position, it was found that G1-like populations developed in both in vitro tumor models. Since the sedimentation velocity technique permits isolation of functionally different cell subpopulations after treatment with cytotoxic agents, it may now be possible to assay differential lethality when such agents are applied to cells growing in situ in a tumor-like situation.
体外培养的中国仓鼠V79 - 171细胞，作为异步单细胞、平台期培养物和多细胞球体时，包含细胞体积不同的亚群。这些亚群可根据其在单位重力下的沉降速度进行分离。发现异步细胞以17.7毫米/小时的模态速度沉降，而平台期细胞和球体细胞的模态沉降速度分别为16.0毫米/小时和15.1毫米/小时。当使用[3H]胸腺嘧啶掺入或对电离辐射的敏感性作为细胞周期位置的指标来确定每个亚群的组成时，发现在两种体外肿瘤模型中都出现了类似G1期的群体。由于沉降速度技术允许在用细胞毒性剂处理后分离功能不同的细胞亚群，现在当将此类药剂应用于类似肿瘤情况的原位生长细胞时，有可能测定差异致死率。
