Davis W L, Jones R G
Tissue Cell. 1984;16(3):443-53. doi: 10.1016/0040-8166(84)90062-4.
Using routine transmission electron microscopy and light and electron microscopic techniques for the histologic demonstration (localization) of catalase (a peroxisomal enzyme), peroxisomes in chick duodenal epithelial cells were identified and studied. In these cells, peroxisomes were seen to be small, ovoid structures, delimited by a single unit membrane. They were concentrated in the supranuclear cytoplasm in initimate association with the smooth endoplasmic reticulum. As demonstrated histochemically, the heterogeneous matrix of these organelles was catalase positive. In addition, most of the larger peroxisomes revealed central nucleoids; however, the smaller peroxisomes were generally anucleoid. It thus appears that two classes of peroxisomes exist in chick intestinal absorptive cells: (1) small, anucleoid microperoxisomes, and (2) larger, nucleoid-containing peroxisomes. In addition to the above morphological characteristics, both peroxisome types were numerous in normal and vitamin-D-replete tissues, but were conspicuously decreased or absent from the apical cytoplasm of rachitic epithelial cells. From these observations it is hypothesized that these organelles may be involved in the overall vitamin-D response of the small intestine.
利用常规透射电子显微镜以及用于过氧化氢酶（一种过氧化物酶体酶）组织学显示（定位）的光镜和电镜技术，对鸡十二指肠上皮细胞中的过氧化物酶体进行了鉴定和研究。在这些细胞中，过氧化物酶体呈小的卵圆形结构，由单层单位膜界定。它们集中在核上区细胞质中，与滑面内质网紧密相连。组织化学显示，这些细胞器的异质性基质呈过氧化氢酶阳性。此外，大多数较大的过氧化物酶体显示有中央核仁；然而，较小的过氧化物酶体通常无核仁。因此，鸡肠道吸收细胞中似乎存在两类过氧化物酶体：（1）小的、无核仁的微过氧化物酶体，以及（2）较大的、含核仁的过氧化物酶体。除上述形态学特征外，这两种类型的过氧化物酶体在正常和维生素D充足的组织中都很多，但在佝偻病上皮细胞的顶端细胞质中明显减少或缺失。从这些观察结果推测，这些细胞器可能参与小肠对维生素D的整体反应。
