Davis W L, Jones R G
Tissue Cell. 1982;14(3):585-95. doi: 10.1016/0040-8166(82)90049-0.
Lysosomes in chick intestinal absorptive cells from rachitic (vitamin D-deficient) and vitamin D-replete animals were studied utilizing transmission electron microscopic histochemistry and ultrastructural morphometry. Absorptive cells from rachitic animals, serum calcium = 7.3 +/- 0.3 mg%, contained an average of 4.0 +/- 0.3 supranuclear lysosomes. In rachitic chicks sacrificed 9 hr post-injection of 1,25-dihydroxycholecalciferol, the active metabolite of vitamin D, the values for both serum calcium, 9.8 +/- 0.2 mg%, and the number of apical absorptive cell lysosomes, 12.9 +/- 0.6, were increased over non-injected or vehicle-only injected animals. Lysosomes in vitamin D-replete absorptive cells were characterized by their intense staining with pyroantimonate, indicative of their high calcium content. The same organelles also produced a positive reaction for acid phosphatase. Rachitic lysosomes, also acid phosphatase positive, were only lightly stained with pyroantimonate. The lysosomal proliferation apparently induced by 1,25-dihydroxycholecalciferol may be a further indication that these organelles play a role in intestinal calcium transport and/or intracellular calcium homeostasis within the absorptive cell.
利用透射电子显微镜组织化学和超微结构形态测定法，对患佝偻病（维生素D缺乏）和维生素D充足的动物的雏鸡肠道吸收细胞中的溶酶体进行了研究。患佝偻病动物的吸收细胞，血清钙含量为7.3±0.3毫克%，平均含有4.0±0.3个核上溶酶体。在注射维生素D的活性代谢物1,25-二羟胆钙化醇9小时后处死的患佝偻病雏鸡中，血清钙含量为9.8±0.2毫克%，顶端吸收细胞溶酶体数量为12.9±0.6，均高于未注射或仅注射溶剂的动物。维生素D充足的吸收细胞中的溶酶体的特征是用焦锑酸盐染色强烈，表明其钙含量高。同样的细胞器对酸性磷酸酶也产生阳性反应。患佝偻病的溶酶体对酸性磷酸酶也呈阳性，但仅用焦锑酸盐轻度染色。1,25-二羟胆钙化醇明显诱导的溶酶体增殖可能进一步表明这些细胞器在吸收细胞的肠道钙转运和/或细胞内钙稳态中起作用。
