几种哺乳动物胸主动脉中糖胺聚糖的比较。

Comparison of glycosaminoglycans from thoracic aortas of several mammals.

作者信息

Aikawa J, Munakata H, Isemura M, Ototani N, Yosizawa Z

出版信息

Tohoku J Exp Med. 1984 May;143(1):107-12. doi: 10.1620/tjem.143.107.

DOI:10.1620/tjem.143.107

Abstract

Intima-media of porcine, canine, rabbit, and human thoracic aortas were separately digested with pronase, after extraction of fat. Glycosaminoglycan fractions were then separated from the resulting glycoconjugate fractions by precipitation with cetylpyridinium chloride. Glycosaminoglycan compositions were determined by sequential digestion with Streptomyces hyaluronidase, chondroitinase AC, chondroitinase ABC and heparitinase 1. All the tissues contained hyaluronic acid, chondroitin sulfates AC, dermatan sulfate and heparan sulfate. Of these glycosaminoglycans, chondroitin sulfates AC were the most prominent ones. Relatively large proportions of heparan sulfate were contained in these tissues except for canine aorta. It is noteworthy that the proportions of glycosaminoglycans in the intima-media of rabbit and human thoracic aortas with similar maturation were quite similar, although those of others differed from each other.

摘要

在去除脂肪后，分别用链霉蛋白酶消化猪、犬、兔和人胸主动脉的内膜中层。然后通过用十六烷基吡啶氯化物沉淀，从所得的糖缀合物组分中分离出糖胺聚糖组分。通过用透明质酸酶、软骨素酶AC、软骨素酶ABC和乙酰肝素酶1顺序消化来测定糖胺聚糖的组成。所有组织均含有透明质酸、硫酸软骨素AC、硫酸皮肤素和硫酸乙酰肝素。在这些糖胺聚糖中，硫酸软骨素AC最为突出。除犬主动脉外，这些组织中含有相对较大比例的硫酸乙酰肝素。值得注意的是，成熟度相似的兔和人胸主动脉内膜中层中糖胺聚糖的比例相当相似，尽管其他组织的比例彼此不同。