Wasserman L, Shlesinger H, Abramovici A, Goldman J A, Allalouf D
Am J Obstet Gynecol. 1980 Dec 1;138(7 Pt 1):763-8. doi: 10.1016/s0002-9378(16)32733-8.
Crude glycosaminoglycan preparations were isolated from acetone-powder of first-, second-, and third-trimester human placentas. Glycosaminoglycan composition was examined by electrophoresis and densitometric scanning before and after treatment with testicular hyaluronidase and chondroitinase ABC. Hexosamines were determined in electrophoretic fractions in order to identify the individual glycosaminoglycans. The results showed a decrease in glycosaminoglycans and an increase in protein contents in the preparations as the placenta matured. The main glycosaminoglycans in all placentas were dermatan sulfate, chondroitin sulfates, hyaluronic acid, and heparan sulfate. With aging of the placenta, the proportion of hyaluronic acid remained unchanged and that of chondroitin sulfate decreased, whereas the proportions of dermatan and heparan sulfates increased. Age-dependent changes in the glycosaminoglycan composition of the placenta appear to follow a pattern similar to that reported for other mammalian tissues.
从妊娠第一、二、三期人胎盘的丙酮粉中分离出粗制糖胺聚糖制剂。在用睾丸透明质酸酶和软骨素酶ABC处理前后，通过电泳和光密度扫描检查糖胺聚糖的组成。测定电泳组分中的己糖胺以鉴定各个糖胺聚糖。结果显示，随着胎盘成熟，制剂中糖胺聚糖减少，蛋白质含量增加。所有胎盘中的主要糖胺聚糖是硫酸皮肤素、硫酸软骨素、透明质酸和硫酸乙酰肝素。随着胎盘老化，透明质酸的比例保持不变，硫酸软骨素的比例下降，而硫酸皮肤素和硫酸乙酰肝素的比例增加。胎盘糖胺聚糖组成的年龄依赖性变化似乎遵循与其他哺乳动物组织报道的模式相似的模式。
