Müller-Klieser W, Vaupel P
Strahlentherapie. 1984 Jul;160(7):436-8.
A pressure chamber for small laboratory animals was designed that can be operated up to 4 bar. It enables the removal of tissue cryobiopsies at liquid nitrogen temperatures during pressurisation. Relevant systemic parameters of the animals investigated, such as mean arterial blood pressure or rectal temperature, can be monitored throughout the experiments. In addition, intravenous infusions of blood, blood substitutes or drugs can be administered. The main goal of constructing the chamber presented is the investigation of tumour oxygenation under hyperbaric conditions. This is performed by measuring the oxyhaemoglobin saturation (HbO2) of single red blood cells in tumour microvessels using a cryophotometric micromethod. In a preliminary series of experiments with rats bearing DS-carcinosarcoma it could be demonstrated that the present experimental set-up leads to HbO2 values in solid tumours which are in accordance with results obtained from previous investigations during exposure to normal air using a different sampling technique.
设计了一种用于小型实验动物的压力室，其工作压力可达4巴。它能够在加压过程中于液氮温度下取出组织冷冻活检样本。在整个实验过程中，可以监测所研究动物的相关全身参数，如平均动脉血压或直肠温度。此外，还可以进行血液、血液替代品或药物的静脉输注。构建该压力室的主要目的是研究高压条件下肿瘤的氧合情况。这是通过使用低温光度微方法测量肿瘤微血管中单个红细胞的氧合血红蛋白饱和度（HbO2）来实现的。在对携带DS-癌肉瘤的大鼠进行的一系列初步实验中，可以证明目前的实验装置所得到的实体瘤中的HbO2值与之前使用不同采样技术在正常空气中暴露期间所获得的结果一致。
