DuVall M D, Ray A C, Reagor J C
Vet Hum Toxicol. 1984 Aug;26(4):293-4.
The effect of different basic drug assay procedures on the formation of artifactual methenamine from formaldehyde preserved and nonpreserved tissue was studied. Trichloroacetic acid was compared with ammonium sulfate as a protein precipitant and sodium hydroxide was compared with ammonium hydroxide as a pH modifier. The assay of nonpreserved tissue failed to result in the detection of methenamine. However, when preserved tissue was assayed by procedures which utilized ammonium compounds, large quantities of methenamine were detected using TLC and GC/MS. Trace quantities of methenamine were detected in extracts of preserved autolyzed tissue which was assayed using nonammonium compounds.
研究了不同的基础药物分析程序对甲醛保存和未保存组织中人为形成乌洛托品的影响。将三氯乙酸与硫酸铵作为蛋白质沉淀剂进行比较，将氢氧化钠与氢氧化铵作为pH调节剂进行比较。对未保存组织的分析未能检测到乌洛托品。然而，当使用铵化合物的程序对保存的组织进行分析时，通过薄层色谱法（TLC）和气相色谱-质谱联用（GC/MS）检测到大量的乌洛托品。在使用非铵化合物分析的保存自溶组织提取物中检测到痕量的乌洛托品。
