Bukrinskiĭ M I, Kramerov D A
Vopr Virusol. 1984 May-Jun;29(3):345-50.
A clone containing the gene of intracisternal A-particles (IAP-gene) was isolated by hybridization with type A double-stranded RNA from the bank of fragments of genome mouse DNA cloned in pBR322 plasmid. Using DNA fragments of this clone we studied transcription of IAP-genes in cells of MOPC-21 plasmocytoma and Ehrlich ascitic carcinoma. Transcription was shown to be partially symmetrical, with predominant transcription of one of DNA chains. The observed transcripts could not be associated with known variants of IAP-genes. The sizes of RNAs transcribed in MOPC-21 and Ehrlich ascitic carcinoma cells and their sequences differed significantly. Apparently, minor variants of IAP-genes are transcribed in the cells under study.
通过与克隆于pBR322质粒的基因组小鼠DNA片段库中的A型双链RNA杂交，分离出了一个包含脑内A颗粒基因（IAP基因）的克隆。利用该克隆的DNA片段，我们研究了MOPC-21浆细胞瘤和艾氏腹水癌细胞中IAP基因的转录情况。结果显示转录部分对称，其中一条DNA链转录占主导。观察到的转录本与已知的IAP基因变体无关。在MOPC-21和艾氏腹水癌细胞中转录的RNA大小及其序列有显著差异。显然，在所研究的细胞中存在IAP基因的微小变体被转录。
