Brown A, Huang R C
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6123-7. doi: 10.1073/pnas.79.20.6123.
EcoRI restriction endonuclease fragments from the mouse 1,350-base-pair EcoRI satellite sequence were cloned in pBR322 plasmid. One of these fragments, SAT-1, was hybridized to various restriction fragments from cloned mouse intracisternal type A particle (IAP) genes and to mouse total genomic DNA. Hybridization occurred between the SAT-1 sequence and each of the IAP clones. The common region of hybridization in each of the clones was the long terminal repeat region of the IAP genes.
从小鼠1350碱基对的EcoRI卫星序列中获得的EcoRI限制性内切酶片段被克隆到pBR322质粒中。其中一个片段SAT-1与克隆的小鼠A型颗粒(IAP)基因的各种限制性片段以及小鼠总基因组DNA进行杂交。SAT-1序列与每个IAP克隆之间均发生了杂交。每个克隆中杂交的共同区域是IAP基因的长末端重复区域。