Hara H, Moriki T, Matsusaki K, Hiroi M, Hashimoto M, Yamane T
Acta Pathol Jpn. 1984 May;34(3):489-97. doi: 10.1111/j.1440-1827.1984.tb07577.x.
The ultracytochemical acridine orange(AO) method has been employed to demonstrate DNA template activity in the developing cerebellar cortex in rats and its alteration after transplacental administration of ethylnitrosourea (ENU). Electron microscopic studies revealed that AO binds to DNA exclusively within the active extended euchromatin portion of the cell nucleus of the proliferating external granular layer. No or only a few AO reaction products were visible in the non-proliferating cell nuclei of the internal granular layer and of Purkinje cells. With increasing cellular differentiation of granule cells in the cerebellar cortex the number of AO positive cells as well as the number of AO reaction products per cell showed a progressive decrease. A single i.p. injection of 50 mg/kg of ENU in rats on day 15 to 21 of gestation resulted in occasional cell damage to the proliferating external granule cells of developing cerebellar cortex at 4 and 7 days after birth. Damaged cells of the external granular layer were found to have no or only a small number of AO reaction products in their nuclei. In further development from 14 to 28 days the cerebellar cortex of offspring from ENU-treated mother showed no abnormality and exhibited no definite alteration in distribution pattern of AO reaction products.
采用超细胞化学吖啶橙（AO）法来证明大鼠发育中小脑皮质的DNA模板活性及其在经胎盘给予乙基亚硝基脲（ENU）后的变化。电子显微镜研究显示，AO仅与增殖的外颗粒层细胞核中活跃伸展的常染色质部分内的DNA结合。在内颗粒层和浦肯野细胞的非增殖细胞核中未见或仅见少数AO反应产物。随着小脑皮质颗粒细胞分化程度的增加，AO阳性细胞的数量以及每个细胞中AO反应产物的数量呈逐渐减少趋势。在妊娠第15至21天对大鼠单次腹腔注射50mg/kg的ENU，导致出生后4天和7天发育中小脑皮质增殖的外颗粒细胞偶尔出现细胞损伤。发现外颗粒层的受损细胞其细胞核中没有或仅有少量AO反应产物。在14至28天的进一步发育过程中，经ENU处理的母亲所生后代的小脑皮质没有异常，并且AO反应产物的分布模式没有明显改变。
