Skikne B S, Linpisarn S, Cook J D
Am J Clin Nutr. 1984 Aug;40(2):346-50. doi: 10.1093/ajcn/40.2.346.
Serum ferritin measurements have become an important laboratory parameter of iron status in prevalence surveys. Because the use of monoclonal antibodies may reduce variability and facilitate standardization, the present study was undertaken to determine whether their performance is comparable to conventional immunological reagents in an immunoradiometric assay for serum ferritin. When evaluated as the capture antibody for the solid phase, no differences in dose response curves were observed between monoclonal reagent and whole antiserum. When monoclonal reagent was used as the radiolabeled indicator antibody, the dose response curves were also similar to those obtained with affinity-purified polyclonal reagent although between-assay variability was somewhat higher with monoclonal antibody. In measurements of serum ferritin in patients with a wide range of iron status, no consistent differences were observed in the results from assays using monoclonal antibody and those of the conventional reagent. These preliminary studies indicate that the performance of monoclonal antibodies in a conventional ferritin assay is comparable to conventional immunological reagents.
在患病率调查中，血清铁蛋白测定已成为铁状态的一项重要实验室参数。由于使用单克隆抗体可能会降低变异性并便于标准化，因此开展了本研究，以确定在血清铁蛋白免疫放射分析中，其性能是否与传统免疫试剂相当。当作为固相捕获抗体进行评估时，单克隆试剂与全抗血清之间未观察到剂量反应曲线的差异。当使用单克隆试剂作为放射性标记指示抗体时，剂量反应曲线也与用亲和纯化多克隆试剂获得的曲线相似，尽管单克隆抗体的批间变异性略高。在对铁状态范围广泛的患者进行血清铁蛋白测量时，使用单克隆抗体的测定结果与传统试剂的结果未观察到一致的差异。这些初步研究表明，在传统铁蛋白测定中，单克隆抗体的性能与传统免疫试剂相当。
