单部位血清铁蛋白免疫放射分析。血清与肝脏铁蛋白标准品及两种抗体制剂的比较。

A single site serum ferritin immunoradiometric assay. A comparison between serum and liver ferritin standards and two antibody preparations.

作者信息

Vidnes A

出版信息

Clin Biochem. 1982 Oct;15(5):252-6. doi: 10.1016/s0009-9120(82)97031-x.

Abstract

A single site immunoradiometric assay (1S-IRMA) for serum ferritin was developed to investigate some standardization problems in the assay of ferritin. In comparison to this 1S-IRMA, a 2-site solid phase IRMA gave lower answers of serum ferritin in the normal reference range, as well as in serum from idiopathic hemochromatosis patients. Purified serum ferritin or liver ferritin gave different standard curves in this new immunoassay system and the differences obtained were further dependent on the antibody preparation employed. The different isoferritin profiles obtained, dependent on the antibody preparation employed for ferritin purification, indicate a different antibody specificity for different ferritin subunits. Serum ferritin purified from different serum pools from healthy persons showed similar isoferritin profiles and immunoassay reactivities.

摘要

为研究铁蛋白检测中的一些标准化问题，开发了一种用于血清铁蛋白的单位点免疫放射分析（1S-IRMA）方法。与这种1S-IRMA相比，双位点固相IRMA在正常参考范围内以及特发性血色素沉着症患者血清中的血清铁蛋白检测结果较低。在这种新的免疫分析系统中，纯化的血清铁蛋白或肝铁蛋白给出了不同的标准曲线，并且获得的差异进一步取决于所使用的抗体制备。根据用于铁蛋白纯化的抗体制备获得的不同异铁蛋白谱，表明针对不同铁蛋白亚基的抗体特异性不同。从健康人的不同血清库中纯化的血清铁蛋白显示出相似的异铁蛋白谱和免疫分析反应性。