Pisciotto P, Kiraly T, Rosen D, Paradis L, Kakaiya R M, Morse E E
Am J Hematol. 1984 Aug;17(2):185-91. doi: 10.1002/ajh.2830170210.
A pheresis procedure was devised to isolate young red cells by centrifugation using the Fenwal CS 3000 continuous flow cell separator. Young red cell enriched products were collected in a 2.5-3-hour procedure. Large numbers of white cells and platelets were collected with the red cells, but cryopreservation and subsequent washing removed 99% of the contaminating cells. At the completion of all processing a product yielding 70% of the total hemoglobin content of a standard frozen/deglycerolized red cell unit was produced. Autologous radiochromium survival of young red cells, measured in 12 normal donors, showed an average 24-hour recovery of 89.9% with a T50Cr of 40.8 days. In paired autologous studies (N = 4) there was a mean increase of 35% in the observed T50Cr of young red cells as compared to standard frozen red cells.
设计了一种单采程序，使用芬华CS 3000连续流动血细胞分离机通过离心分离年轻红细胞。在2.5至3小时的程序中收集富含年轻红细胞的产品。大量白细胞和血小板与红细胞一起被收集，但冷冻保存和随后的洗涤去除了99%的污染细胞。在所有处理完成时，产生了一种产品，其血红蛋白含量占标准冷冻/去甘油红细胞单位总血红蛋白含量的70%。对12名正常供体进行的年轻红细胞自体放射性铬存活测量显示，平均24小时回收率为89.9%，T50Cr为40.8天。在配对自体研究（N = 4）中，与标准冷冻红细胞相比，观察到的年轻红细胞T50Cr平均增加了35%。
