猪血浆胺氧化酶催化循环过程中氨释放的动力学
The kinetics of ammonia release during the catalytic cycle of pig plasma amine oxidase.
作者信息
Rius F X, Knowles P F, Pettersson G
出版信息
Biochem J. 1984 Jun 15;220(3):767-72. doi: 10.1042/bj2200767.
Abstract
The time course of NH3 release during the catalytic cycle of pig plasma amine oxidase was followed by using the quenched-flow technique in conjunction with a sensitive assay for NH3. These studies were made under both air and O2-saturating conditions. The results establish unequivocally that NH3 is released in the step whereby a reduced enzyme species is re-oxidized by molecular O2 rather than in the step leading to the reduced enzyme. It is concluded that the catalytic cycle of the enzyme conforms to an aminotransferase mechanism rather than one in which an imine is an intermediate.
摘要
采用猝灭流动技术结合灵敏的氨检测方法，追踪猪血浆胺氧化酶催化循环过程中氨的释放时间进程。这些研究是在空气和氧气饱和条件下进行的。结果明确证实，氨是在还原型酶物种被分子氧重新氧化的步骤中释放出来的，而不是在导致还原型酶的步骤中释放。结论是，该酶的催化循环符合转氨酶机制，而不是以亚胺为中间体的机制。
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