Puchkov V F
Arkh Anat Gistol Embriol. 1978 Feb;74(2):50-4.
The inhibitor of protein synthesis chloramphenicol (2 mg/egg) was applied to elucidate the critical period in the chicken lens development. Chloramphenicol injected before incubation and at 24, 30 and 48 h of incubation did not prevent the formation of parts in the lens-inducing apparatus (the optic vesicle, and the presumptive lens ectoderm), but the injection at the stage of 24--30 h of incubation resulted, in many survived for 5--7 days of incubation, in lack of the lens. Therefore, it is possible to speak about a disturbance in the activity of the inducing apparatus during the period of determination, or about a critical period of the lens development.
应用蛋白质合成抑制剂氯霉素(2毫克/枚鸡蛋)来阐明鸡晶状体发育的关键时期。在孵化前以及孵化24、30和48小时时注射氯霉素,并未阻止晶状体诱导装置(视泡和假定的晶状体外胚层)各部分的形成,但在孵化24至30小时阶段注射氯霉素,导致许多在孵化5至7天存活下来的胚胎没有晶状体。因此,可以认为在决定期诱导装置的活性受到了干扰,或者说存在晶状体发育的关键时期。